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Aquarium Fish Safety

Important Bulletin for Aquarium Owners

Due to the many variables possible with respect to the safety of aquatic life, there is no simple way to declare epoxy completely safe for all aquatic life. AAI does not warranty that your fish will remain healthy if you use our adhesive.

With respect to the question of fish safety, AAI hired a laboratory to conducted a test (DOHS (Title 22) Hazardous Waste Bioassay using Flathead Minnows ) which was passed that relates to the exposure of materials like our epoxy in a marine environment. Please note that it is important to fully mix the two parts of the epoxy above water per the directions supplied with the epoxy before applying underwater. The mixed solution's safety is what is reflected in the test.

Mr. Sticky's® Underwater Glue has passed the "DOHS (Title 22) Hazardous Waste Bioassay using Flathead Minnows test: This test is specified under the California Department of Fish and Game's regulations. Please review it and decide for yourself if it is sufficient to warrant usage in your situation.

Test Results

Acute Bioassay Report

Performed by Aquatic Bioassay and Consulting Laboratories, Inc. in Ventura, CA, on

May 25, 2005.

The test was conducted under the guidelines prescribed in “Static Acute Bioassay

Procedures for Hazardous Waste Samples” California Department of Fish and Game,

1998. The results were as follows:

Sample I.D.: Composite Hardener and Resin

DOHS (TITLE 22) HAZARDOUS WASTE BIOASSAY USING FATHEAD

MINNOWS

96 HOUR LC50 = >750 mg/l

STATUS = Pass

WATER QUALITY

Beg: 49 mg/l End: 52 mg/l Beg 37 mg/l End: 39 mg/l

Beg: 52 mg/l End: 55 mg/l Beg: 33mg/l End: 33mg/l

SAMPLE HARDNESS SAMPLE ALKALINITY

DILUTION WATER: Reconst. Fresh AERATION: Single Bubble Air

CONTROL HARDNESS CONTROL ALKALINITY

ORGANISM INFORMATION

Pimephales promelas DATE REC'D: 5/6/2005

COMMON NAME: Fathead Minnow AVERAGE LENGTH: 39mm

Thomas Fish Co. AVERAGE WT: .77 gm

California Overnight NO. FISH/TANK: 10

SPECIES:

SOURCE:

CARRIER:

TEST DATA

Date

Time

Conc. Dis. Oxy. Temp (C.) pH Dis. Oxy. Temp (C.) pH # fish dead

0 (con.) 8.9 20.9 7.5 7.2 20.4 8 0

400 mg/l 8.9 20.9 7.9 8.0 19.8 7.3 0

400 mg/l 8.9 20.9 7.9 8.1 19.6 7.4 0

750 mg/l 8.9 20.9 7.9 7.9 19.6 7.4 0

750 mg/l 8.9 20.9 7.9 7.8 19.7 7.3 0

1700 1400

INITIAL 24 HOURS

5/25/2005 5/26/2005

Dis. Oxy. Temp (C.) pH # fish dead Dis. Oxy. Temp (C.) pH # fish dead

7 19.9 7.3 0 7 19.7 7.3 0

7.8 19.4 7.1 0 7.7 19.6 7.1 0

7.6 19.3 7.1 0 7.8 19.6 7.1 0

7.7 19.3 7 0 7.8 19.6 7.1 0

7.3 19.5 7 0 7.7 19.6 7.1 0

1800 1500

48 HOURS 72 HOURS

5/27/2005 5/28/2005

Dis. Oxy. Temp (C.) pH # fish dead

6.8 19.5 7.3 0

7.6 19.7 7.1 0

7.5 19.7 7.1 0

7.5 19.7 7.1 0

7.5 19.7 7.1 0

96 HOURS

5/29/2005

1600

FINAL DATA

TOTAL MORTALITIES

0 (Con.) 0

400 mg/l 0

400 mg/l 0

750 mg/l 0

750 mg/l 0

FINAL RESULTS

>750 mg/l

Pass

Binomial Test

FINAL RESULTS

96 HOUR LC50 =

STATUS =

CALCULATION METHOD =